开元棋牌电脑 |本期目录/Table of Contents|

[1]代闫芳,刘素素,岳娟,等.双醋瑞因减轻小鼠角膜碱烧伤炎症反应的实验研究[J].开元棋牌电脑_开元棋牌一直在维护_开元棋牌怎么对刷,2019,39(4):321-326.[doi:10.13389/j.cnki.rao.2019.0072]
 DAI Yan-Fang,LIU Su-Su,YUE Juan,et al.Diacerein alleviates the inflammatory response of mice following corneal alkali burn[J].Recent Advances in Ophthalmology,2019,39(4):321-326.[doi:10.13389/j.cnki.rao.2019.0072]
点击复制

双醋瑞因减轻小鼠角膜碱烧伤炎症反应的实验研究(/HTML)
分享到:

《开元棋牌电脑_开元棋牌一直在维护_开元棋牌怎么对刷》[ISSN:1003-5141/CN:41-1105/R]

卷:
39卷
期数:
2019年4期
页码:
321-326
栏目:
实验研究
出版日期:
2019-04-05

文章信息/Info

Title:
Diacerein alleviates the inflammatory response of mice following corneal alkali burn
作者:
代闫芳刘素素岳娟简守珺张芬芬魏静静司玮张红敏
450003 河南省郑州市,郑州大学人民医院、河南省立眼科医院、河南省眼科研究所、河南省眼科学与视觉科学重点实验室
Author(s):
DAI Yan-FangLIU Su-SuYUE JuanJIAN Shou-JunZHANG Fen-FenWEI Jing-JingSI WeiZHANG Hong-Min
People’s Hospital of Zhengzhou University,Henan Eye Hospital,Henan Eye Institute,Henan Key Laboratory of Ophthalmology and Vision Science,Zhengzhou 450003,Henan Province,China
关键词:
双醋瑞因角膜碱烧伤混浊度病灶面积炎症细胞
Keywords:
diacereincorneal alkali burnopacitypathological areainflammatory cells
分类号:
R772
DOI:
10.13389/j.cnki.rao.2019.0072
文献标志码:
A
摘要:
目的 探讨双醋瑞因减轻小鼠角膜碱烧伤炎症反应的作用。方法 将174只小鼠角膜碱烧伤造模成功的小鼠随机分为三组:双醋瑞因组、地塞米松组和生理盐水组,分别给予双醋瑞因、地塞米松、生理盐水滴眼制剂滴眼,比较造模后2 d、3 d、4 d、5 d各组小鼠的角膜混浊度、病灶面积、上皮缺损面积及炎症细胞的浸润情况。结果 双醋瑞因组造模后4 d、5 d角膜临床评分明显较生理盐水组低(均为P<0.01),地塞米松组造模后3 d、4 d、5 d角膜临床评分较生理盐水组低(均为P<0.05)。对于角膜上皮缺损而言,双醋瑞因组碱烧伤后各时间点角膜上皮缺损面积均小于生理盐水组(均为P<0.05),地塞米松组与生理盐水组角膜上皮缺损无明显差异。对角膜中性粒细胞进行统计发现,双醋瑞因组碱烧伤后各时间点中性粒细胞计量明显少于生理盐水组(均为P<0.01);地塞米松组造模后3 d、5 d中性粒细胞浸润少于生理盐水组(均为P<0.05)。对巨噬细胞进行统计发现,双醋瑞因组造模后3 d、5 d巨噬细胞均较生理盐水组浸润少(均为P<0.05);造模后5 d地塞米松组浸润少于生理盐水组(P<0.05)。通过MPO活性测定发现,双醋瑞因组和地塞米松组造模后2 d、3 d、5 d MPO活性均较生理盐水组明显降低(均为P<0.01)。结论 双醋瑞因能够减轻小鼠角膜碱烧伤的炎症反应,改善角膜碱烧伤的预后。
Abstract:
Objective To investigate the effect of diacerein on reducing inflammation of mice after corneal alkali burn.Methods After successful establishment of corneal alkali burn model,174 mice were divided into three groups:diacerein group,dexamethasone group and the saline group,which were given diacerein,dexamethasone and saline eye drops intervention,respectively.The opacity of corneal,pathological area,epithelial defect area,the expression of inflammatory cell were compared between the groups on day 1,2,3,4,and 5 after modeling.Results The clinical scores were significantly lower in the diacerein group than in the saline group on day 4 and 5 (all P<0.01),and the dexamethasone group was lower than the saline group on day 3,4 and 5 after modeling (all P<0.05).For corneal epithelial defects,the diacerein group was smaller than the saline group at each time point (all P<0.05),and there was no significant difference between the dexamethasone group and saline group.Statistical analysis showed that the number of neutrophils was significantly lower in the diacerein group than in the saline group at each time point (all P<0.01),and the dexamethasone group was less than the saline group on day 3 and 5 (both P<0.05).According to the statistics of macrophages,the diacerein group was less than the saline group on day 3 and 5 after modeling (both P<0.05),and the dexamethasone group was less than the saline group on day 5 (P<0.05).From the results of myeloperoxidase (MPO) activity assays,the MPO activity of both the diacerein group and dexamethasone group was significantly lower than that of the saline group at each point time after modeling (all P<0.01).Conclusion Diacerein can reduce the inflammatory response and improve the prognosis of mice following corneal alkali burn.

参考文献/References:

[1] MOHAN G C,ZHANG H Y,BAO L,MANY B,CHAN L S.Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells[J].PLoS One,2017,12(3):e0173981.
[2] FIDELIX T S A,MACEDO C R,MAXWELL L J,FERNANDES Moc Trevisani V.Diacerein for osteoarthritis[J].Cochrane Database Syst Rev,2014,(2):CD005117.
[3] DOU X Y,CHEN S W,YANG K,JIN X,ZHANG Z R,ZHANG H M.Pharmacokinetic behaviors of diacerein in mouse corneas[J].Chin Trad Patent Med,2017,39(11):2289-2292.
窦新岩,陈世伟,杨柯,金鑫,张晊瑞,张红敏.双醋瑞因在小鼠角膜中的药代动力学行为[J].中成药,2017,39(11):2289-2292.
[4] SHARMA N,KAUR M,AGARWAL T,SANGWAN V S,VAJPA-YEE R B.Treatment of acute ocular chemical burns[J].Surv Ophthalmol,2018,63(2):214-235.
[5] SHIN Y J,HYON J Y,CHOI W S,YI K,CHUNG E S,CHUNG T Y,et al.Chemical injury-induced corneal opacity and neovascularization reduced by rapamycin via TGF-β1/ERK pathways regulation[J].Invest Ophthalmol Vis Sci,2013,54(7):4452-4458.
[6] CHOI H,PHILLIPS C,OH J Y,CHOI H,PHILLIPS C.Comprehensive modeling of corneal alkali injury in the rat eye[J].Curr Eye Res,2017,42(10):1348-1357.
[7] ZHANG H M,LIU S S,XU Z Z,MA C H,XIE Y T,WU X P,et al.Effects of immunocyte on the process of fungal keratitis[J].Chin J Exp Ophthalmol,2012,30(9):779-784.
张红敏,刘素素,许中中,马聪慧,谢艳亭,吴细丕,等.小鼠真菌性角膜炎中主要免疫细胞的作用[J].中华实验眼科杂志,2012,30(9):779-784.
[8] PASIN J S,FERREIRA A P,SARAIVA A L,RATZLAFF V,ANDRIGHETTO R,TOMAZETTI J,et al.Diacerein decreases TNF-alpha and IL-1beta levels in peritoneal fluid and prevents Baker’s yeast-induced fever in young rats[J].Inflamm Res,2010,59(3):189-196.
[9] HAMILL C E,BOZORG S,PEGGY CHANG H Y,LEE H,SAYEGH R R,SHUKLA AN,et al.Corneal alkali burns:a review of the literature and proposed protocol for evaluation and treatment[J].Int Ophthalmol Clin,2013,53(4):185-194.
[10] PATTAMATTA U,WILLCOX M,STAPLETON F,GARRETT Q.Bovine lactoferrin promotes corneal wound healing and suppresses IL-1 expression in alkali wounded mouse cornea[J].Curr Eye Res,2013,38(5):1110-1117.
[11] LI Z,CUI L,YANG J M,LEE H S,CHOI J S,WOO J M,et al.The wound healing effects of adiponectin eye drops after corneal alkali burn[J].Curr Eye Res,2016,41(7):1424-1432.
[12] TOEGEL S,WU S Q,PIANA C,UNGER M F,WIRTH M,GOLDRING B M,et al.Comparison between chondroprotective effects of glucosamine,curcumin,and diacerein in IL-1beta-stimulated C-28/I2 chondrocytes[J].Osteoarthr Cartil,2008,16(6):1205-1212.
[13] CHEN Y,YANG W,ZHANG X,YANG S,PENG G,WU T,et al.MK2 inhibitor reduces alkali burn-induced inflammation in rat cornea[J].Sci Rep,2016,6:28145.
[14] HUA X,YUAN X Y,LI Y H,CHEN H,YUAN J,TANUMIHARJO S V,et al.Desiccating stress worsens alkali burn injury by magnifying caspase-8-induced imbalance of NLRP3 and NLRP6[J].J Allergy Clin Immunol,2017,140(4):1172-1176.
[15] BIAN F,XIAO Y,ZAHEER M,VOLPE E A,PFLUGFELDER S C,LI D Q,et al.Inhibition of NLRP3 inflammasome pathway by butyrate improves corneal wound healing in corneal alkali burn[J].Int J Mol Sci,2017,18(3):E562.
[16] LIU S H,MA K,XU X R,XU B.A single dose of carbon monoxide intraperitoneal administration protects rat intestine from injury induced by lipopolysaccharide[J].Cell Stress Chaperones,2010,15:717-27.
[17] HAN Y,SHAO Y,LIN Z,QU Y L,WANG H,ZHOU Y,et al.Netrin-1 simultaneously suppresses corneal inflammation and neovascularization[J].Invest Ophthalmol Vis Sci,2012,53(3):1285-1295.
[18] SHAEIB F,KHAN S N,THAKUR M,KOHAN-GHADR HR,DREWLO S,SAED G M,et al.The impact of myeloperoxidase and activated macrophages on metaphase II mouse oocyte ouality[J].PLoS One,2016,11:e0151160.
[19] CHIEN K J,YANG M L,TSAI P K,SU C H,CHEN C H,HORNG C T,et al.Safrole induced cytotoxicity,DNA damage,and apoptosis in macrophages via reactive oxygen species generation and Akt phosphorylation[J].Environ Toxicol Pharmacol,2018,64:94-100.

相似文献/References:

[1]赵旭东 高晓唯 李文静 刘莹 周昕 杨磊.胶原交联对兔角膜碱烧伤后基质融解的影响[J].开元棋牌电脑_开元棋牌一直在维护_开元棋牌怎么对刷,2012,32(6):.
[2]吴利安,王从毅,杨新光,等. 骨髓间充质干细胞移植对兔碱烧伤角膜新生血管形成的抑制作用[J].开元棋牌电脑_开元棋牌一直在维护_开元棋牌怎么对刷,2015,35(7):623.[doi:10.13389/j.cnki.rao.2015.0169]
 WU Li-An,WANG Cong-Yi,YANG Xin-Guang,et al. Inhibitive effects of bone marrow stem cells on corneal neovascularization in rabbit with corneal alkali burn[J].Recent Advances in Ophthalmology,2015,35(4):623.[doi:10.13389/j.cnki.rao.2015.0169]

备注/Memo

备注/Memo:
国家自然科学基金资助(编号:81770902);河南省自然科学基金资助(编号:162300410163);河南省科技创新人才计划项目(编号:144100510015)
更新日期/Last Update: 2019-04-15